Scientifica HyperScope
Push the boundaries of light microscopy and find the answers to the most intricate biological questions with the HyperScope, a proven multiphoton microscope.
Image using two- and three-photon fluorescence excitation with exceptional performance. The HyperScope multiphoton imaging system is a trusted solution that can be customised and upgraded to suit your changing needs. The option of a dual scan head allowing for simultaneous imaging and photostimulation enables you to probe the functional roles of neural cells and circuits.
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Tailor your HyperScope to suit your needs and budget
Available with a variety of options for scan head, frame and detector, the HyperScope can be tailored to suit your needs. Existing components in your lab can also be used to build a HyperScope multiphoton imaging system within your budget. This ensures your research is not limited by your funding.
With easily removable covers, the light paths are accessible for customisation. When in the single scanhead configuration, the second mounting position offers a standardised optomechanical interface where visual stimulus and other custom scan paths can be added.
The ability of the HyperScope to combine imaging with other techniques, such as electrophysiology, means your experiments have no limit.
Tailor your HyperScope to suit your needs and budget
Available with a variety of options for scan head, frame and detector, the HyperScope can be tailored to suit your needs. Existing components in your lab can also be used to build a HyperScope multiphoton imaging system within your budget. This ensures your research is not limited by your funding.
With easily removable covers, the light paths are accessible for customisation. When in the single scanhead configuration, the second mounting position offers a standardised optomechanical interface where visual stimulus and other custom scan paths can be added.
The ability of the HyperScope to combine imaging with other techniques, such as electrophysiology, means your experiments have no limit.
An all-in-one solution for imaging and stimulation
Push the boundaries of light microscopy with the HyperScope, the ideal solution for using the following techniques to investigate biology:
- In vivo and in vitro two-photon imaging
- Three-photon imaging
- Second and third harmonic generation
- Two-photon photostimulation
- Fluorescence lifetime imaging
- All-optical interrogation of neurons with the HoloStim-3D.
A trusted solution for in vivo and in vitro two-photon imaging
Obtain images from both in vivo and in vitro samples with high spatial resolution to answer novel biological questions. The flexibility of the HyperScope enables it to be easily adapted to suit your experimental requirements, ensuring the raw data is high quality, so less processing and analysis is required.
With the option of a tiltable objective, you can retain optimal alignment during in vivo experiments, without having to tilt the animal. Easily switch between a tiltable objective and regular objective mount, for optimal and cost-effective experimental flexibility.
Imaged during the Drosophila course at Cold Spring Harbor in 2017
Z-stacks from in vivo 6 days post fertilisation zebrafish
Two-photon recording from a three week old zebrafish brain explant of the line Tg(elavl3:GCaMP6s-nuclear). Four consecutive planes (upper to lower ones from left to right) of a volumetric recording (8 planes in total) are shown where first Dm (amygdala homolog in zebrafish) then Dl (Hippocampus homolog in zebrafish) were electrically stimulated by a glass-electrode (indicated in the upper left corner). Data acquired by Anna Ostenrath and Ewelina Bartoszek, PhD Students in the lab of Professor Emre Yaksi, Kavli Institute, NTNU.
From left to right: Bovine pulmonary artery endothelial cells labelled with MitoTracker® Red CMXRos for mitochondia, stained with Alexa Fluor® 488 phalloidin for F-actin; Muntjac skin fibroblast, F-actin was labelled with green-fluorescent Alexa Fluor® 488 phalloidin; Mouse kidney section stained with Alexa Fluor® 488 wheat germ agglutin - the filamentous actin prevalent in glomeruli and the brush border were stained with red-fluorescent Alexa Fluor® 568.
The underside of an American Oak leaf, imaged using two-photon excitation. Single focus plane from a 3D image projection of the entire imaged volume. Captured using Scientifica's Hyperscope system at Cold Spring Harbour in 2017.
Stereo image of intact ex vivo central nervous system of a Drosophila melanogaster larva with GCaMP6m expressed in all motor neurons. Image acquired on a Hyperscope with James Macleod, with support from Prof. Stefan Pulver and CSHL.
Case studies using the Scientifica HyperScope
Retinotopy in Mouse Superior Colliculus Mapped with Single Cell Precision using HyperScope
A published paper in Nature by researchers from the Epigenetics and Neurobiology Unit at the European Molecular Biology Laboratory used the Scientifica HyperScope for in vivo two-photon imaging, mapping with single-cell precision the retinotopy in mouse superior colliculus. Read more here.
The Scientifica HyperScope was used by researchers at the Thibault Lab, University of Kentucky, to investigate in vivo neuronal calcium dysregulation in primary somatosensory cortex (S1) associated with age-dependent changes in locomotor stability. Read more here
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