CoolLED pE-300lite
A system that offers intense, broad-spectrum LED illumination for imaging most common fluorescent stains
The light source can be fitted directly to the microscope or specified with a liquid light guide. The system is a mercury-free alternative that is safer, more controllable, and repeatable than conventional high-pressure gas discharge illuminators. The system is perfect for hospital and regular research laboratory environments and is affordable within consumables budgets.
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System overview
The system comprises a pE-300lite Light Source, control pod, and power supply. It can be specified with a microscope adaptor for direct fit configuration or with a 3 mm diameter liquid light guide if there is a need to keep the source of illumination remote from the microscope. An optional pE-Universal Collimator and microscope adaptor can be selected for use with a liquid light guide.
As a replacement to a metal halide source on a confocal microscope, the liquid light guide configuration is particularly suitable for pre-aligning samples before scanning commences. In this way, photo bleaching of the sample can be minimised when LED illumination is used.
The pE-300lite provides a broad spectrum of illumination
Enhanced spectrum
The pE-300lite provides a broad spectrum of illumination, covering the excitation bands of many common fluorescent stains. Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%.
Life expectancy of the pE-300lite
Long lasting
With an expected lifetime in excess of 25,000 on hours and a comprehensive range of microscope adaptors, the pE-300lite can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.
Choose single-band or multi-band
DAPI can be excited at either 365 nm or 400 nm and microscopes typically have a filter set which allows one or other of these wavelengths to be transmitted. To accommodate this, the pE-300lite can be specified in one of two waveband configurations to match existing filter sets within the microscope.
A microscope which is populated with a number of single band (“SB”) filter sets will typically have DAPI excitation at 365 nm and a microscope with multi-band (“MB”) filter(s) has excitation at 400 nm. The user can specify the configuration which is appropriate for their microscope.
Configuration Options
Direct-fit for connecting to a microscope – by selecting from a range of microscope adaptors which covers all current and most older models. A simple once only adjustment will allow optimisation to the optical path of the microscope.
Liquid Light Guide with a fixed 3 mm diameter, liquid light guide. An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required.
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